Non-canonical targets destabilize 토토 사이트 순위s in human Argonautes

토토 사이트 순위 is short 20-22-nucleotide RNA molecules. Unlike the messenger RNA (mRNA), 토토 사이트 순위 is a type of non-coding RNA that is not translated into protein. These 토토 사이트 순위s bind complementarily to the mRNA sequence through the 2nd to 7th "seed site" from the 5' terminal of the 토토 사이트 순위 sequence and inhibit the synthesis of proteins encoded by the mRNA. Therefore, 토토 사이트 순위s serve as negative regulators of gene expression.

The biochemistry laboratory led by Professor Shin, Chanseok of the College of Agriculture and Life Science at Seoul National University is investigating the mechanisms by which the 토토 사이트 순위 recognizes and inhibits its target mRNA, and the expression control mechanisms of 토토 사이트 순위s in plants and animals.

Target recognition by 토토 사이트 순위s in animals begins with the loading of 토토 사이트 순위s into Argonaute (AGO)-containing effector complexes called RNA-induced silencing complex (RISC) that recognizes the complementary mRNA sequence. This 토토 사이트 순위-mRNA binding through the "seed site" is called a canonical binding. Sequences other than the 토토 사이트 순위 "seed site" also bind with mRNA sequences. Such binding where the “seed site” is incomplete are called “non-canonical binding.” Although these non-canonical targets are frequently found in cells, there is not yet enough research on how these non-canonical targets function in terms of regulating the expression of 토토 사이트 순위s or targeting mRNAs.

Professor Shin’s research team examined the mechanism of the 토토 사이트 순위 3’ terminal destabilization phenomenon via non-canonical bindings in animal 토토 사이트 순위s using a variety of in vitro assays using biochemical techniques. When a 토토 사이트 순위 binds to a non-canonical targets, a conformational change may occur in the AGO protein. As a result, the 3’ terminal of the 토토 사이트 순위 is released from the AGO protein and thus susceptible to degradation by 3’-5’ exonuclease. ..

These results were published in the famous international journal Nucleic Acids Research (NAR) in February of this year under the title “Non-canonical Targets Destabilize 토토 사이트 순위s in Human Argonautes.” It was listed for the first time as a 2017 breakthrough article (a report selected by NAR regarding an eye-opening phenomenon in the field or a paper on answering important questions in the relevant field.).

Professor Shin praised the results of the study by saying “whether or not mRNA expression is inhibited through the 토토 사이트 순위 often has a great influence in terms of various vital phenomena” and that “the 토토 사이트 순위’s 3’ terminal destabilization mechanism through non-canonical binding is a type of risk management mechanism to minimize target misrecognition from incorrect 토토 사이트 순위 binding and the resulting vital phenomenon malfunctions”. Therefore, he concluded that the results of this study is an “aspect of the “RISCy” business of 토토 사이트 순위. (Here, RISCy has the same pronunciation as “risky” and refers to the RISC complex that is triggered by a 토토 사이트 순위.)

Regarding the direction of future research, Professor Shin ended the interview by stating, “There must be more in-depth research on the detailed biological significance of the 토토 사이트 순위 expression control phenomenon through these mechanisms under the actual physiological contexts, such as development, differentiation, and disease.”

Student Reporter Song, Iee-re / Seong, Gayeong

Shin's Research Team